Laser Scanning Confocal Microscopy: Application in Manufacturing and Research of Corneal Stem Cells

In parallel, the development and manufacturing of fluorescent probes (=fluorophores) characterized by low toxicity profiles are allowing to perform the above mentioned studies using living cell cultures or tissues that are not fixed. Furthermore, fluorescent proteins such as the Green Fluorescent Protein (GFP) and its derivatives allow to detect how the biosynthetic machinery of the cell works or a transgene (driven by a plasmid or a genetically engineered virus) is expressed (Figure 2) or a chimeric protein interacts with other cellular components.